Composite

Part:BBa_K1997022:Design

Designed by: Chushu Zhu   Group: iGEM16_NUDT_CHINA   (2016-10-13)


P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1609
    Illegal AgeI site found at 1693
    Illegal AgeI site found at 1813
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1816
    Illegal BsaI.rc site found at 1513


Design Notes

We added BsaI sites at the flank of "Zif-N->RBS->Zif-C" sequence so that this sequence can be easily replaced


Source

This part was synthesized by IDT

References